Determination Of Human Papilloma Virus (HPV) Using Cytomorphology, Immuno cytochemistry and In situ-hybridization In Cervical Smears Of married Women in Khartoum State Sudan 2006

Abstract

Abstract:

This descriptive comparative study was aims to measure the sensitivity and specificity of cytological, immunocytochemical and in situ−hybridization detection methods for Human Papilloma Virus (HPV) in cervical smear. Ninety seven cervical smears were collected from married females with different gynecological complains and twenty one of them were well diagnosed with cervical cancer. In cervical smears , HPV classical cytomorphological changes (Koilocytosis andƒor dyskeratosis) were detected in 36% while HPV K1−h 8 anUgen was detected in 19% by immunocytochemistry staining method and HPV DNA was detected in 51% by in situ−hybridization technique . HPV DNA was detected in 90% of ladies with cervical cancer smears and in 39% of ladies with other complains smears. Using in situ− hybridization as a gold standard test cytology has a sensitivity of 67.3% and specificity of 85.5%. Immunocytochemistry has a sensiUvity of 36.7%, a specificity of 97.7%. This study concludes that cytomorphological screening for HPV in cervical smears is suitable for clinical practical purposes in compare to antigen and DNA detection method.