Assessment of Proliferation Activity by Using Nucleolar Organiser Regions Count among Sudanese Patients with Prostate Cancer and Benign Prostate Hyperplasia

Abstract

Background Prostatic adenocarcinoma is the most prevalent cancer and the second cause of cancer related death among men; the tumour proliferative activity is difficult to measure histologically. Increasing evidence suggests that the factors controlling cell cycle progression also modulate the rate of ribosome biogenesis; and can assess the proliferative activity. The present study aimed to assess the proliferation activity in prostate cancer. Materials and Methods A total of 40 various prostatic lesions were studied, 20 cases of prostatic adenocarcinomas (study group) and 20 cases of benign prostatic hyperplasia (BPH) as (control group). Sections of 3-µ thickness was obtained from each formalin-fixed paraffin-embedded tissue block using rotary microtome and it was stained using haematoxylin and eosin (Mayer’s technique) and AgNOR stains. results The majority of patients with BPH and prostate adenocarcinoma were in their sixth to eighth decade of life. The BPH samples displayed fewer AgNORs (mean 2.0 dots/cell) compare to adenocarcinomas (mean 4.1 dots/cell), p value was (0.001). Therefore this data indicate that analysis of silver staining-positive material in intact interphase cells may help distinguish between benign and malignant prostatic tumours. conclusions AgNOR have a value in distinguishing between BPH and adenocarcinoma of the prostate.