Molecular screening for Ty-1, Ty-2, Ty-3, Ty-4 and Ty-5 genes in two tomato breeding lines

Abstract

Tomato yellow leaf curl virus (TYLCV) is a major tomato virus disease in tropical and subtropical regions. Ty-1, Ty-2, Ty-3, Ty-4 and Ty-5 are genes conditioning resistance to TYLCV disease in tomato. Molecular screening for these genes in two tomato breeding lines resistant to TYLCV disease (Multichiltylc-95-J0-C2 and Pimpertylc-J-13) was done using five sequence tagged site (STS) markers included (TG178/Taq1), (T0302), (FLUW25), (P3-81/Mas1) and (SINAC1/Taq1) which linked to Ty-1, Ty-2, Ty-3, Ty-4 and Ty-5 respectively. Five TYLCV resistant lines in addition to a susceptible one were used as checks. The experiment was conducted at Biotechnology and Molecular Breeding Unit of the Asian Vegetable Research Development Center (AVRDC), the World Vegetable Center in Taiwan. Results indicated that Multichiltylc-95-J0-C2 did not carry Ty-1, Ty-2, Ty3, Ty-4 or Ty-5 genes. Pimpertylc-J-13 did not carry Ty-1, Ty-2, Ty3, Ty-4 but it appeared that it may carry Ty-5, a resistance gene from Solanum peruvianum.