Characterization of protein in the secretions of paratoid gland of toads (Amietophrynus spp) collected from shendi area

Abstract

Abstract The parotoid glands secretions of two species, namely Amietophrynus regularis and Amietophrynus xeros, were investigated by Bradford estimation test for determining the concentration of proteinaceous compound in the secretions. The results revealed a wide range of proteins that differed between the two species. The average of protein content in A. regularis secretions was estimated as 26.95± 8.89% while the A. xeros was 23.86± 8.94 %. Statistical test by F test and t student test illustrated significant difference between the two groups of protein concentrations. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDSPAGE) illustrated two different protein profiles for the two species that indicated the evolutionary and taxonomical value of the paratoid secretions. Furthermore it revealed different protein profiles within each species, indicating the existence of subspecies or the existence of another species that was extremely similar to the species, especially in the case of Amietophrynus regularis. The Retention factor (Rf) and molecular weights of the protein