Detection of Human Papilloma Virus Type 16 and 18 E6 Early Protein among Sudanese Esophageal Tumors Patients using Immuno histochemistry

Abstract

Esophageal cancer is the eight most common cancer worldwide and the sixth cause of cancer related death with squamous cell carcinoma (SCC) accounting for almost half of all esophageal cancers. Oncogenic human papilloma virus (HPV) has been hypothesized as a risk factor for esophageal squamous cell carcinoma. The aims of this review was to estimate the present of (HPV 16 and 18) by immuno histochemical technique In this is descriptive retrospective study, which carried out in Ibn sena HospitalKhartoum-Sudan, formalin fixed paraffin embedded blocks from 30 patients previously diagnosed as esophageal tumors were included (20 of them with esophageal malignant tumor and 10 of them were benign) ,their age ranging from 8-98 years with mean age of 59 years old Five (4) micron was taken on positively coated slide for immuno histochemical detection of HPV (16 and 18). SPSS version 11.5 computer programs were used to analyze the data, frequencies, and means, the P. value was calculated by Chi square test. The study involved (30) subject twenty out of them were males (66.7%) and (33.3%) were females with (2:1) male: female ratio. Sven samples (23.3%) were positive for HPV (16, 18), while 23(76.7%) were negative, with no statistically significant difference between HPV infection and type of tumors. HPV infection, especially type 16, 18 should be considered as a risk factor for esophageal malignancies in Sudan.