Abstract:
Abstract
The parotoid glands secretions of two species, namely
Amietophrynus regularis and Amietophrynus xeros, were investigated by
Bradford estimation test for determining the concentration of
proteinaceous compound in the secretions. The results revealed a wide
range of proteins that differed between the two species. The average of
protein content in A. regularis secretions was estimated as 26.95± 8.89%
while the A. xeros was 23.86± 8.94 %. Statistical test by F test and t
student test illustrated significant difference between the two groups of
protein concentrations.
Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDSPAGE) illustrated two different protein profiles for the two species that
indicated the evolutionary and taxonomical value of the paratoid
secretions. Furthermore it revealed different protein profiles within each
species, indicating the existence of subspecies or the existence of
another species that was extremely similar to the species, especially in
the case of Amietophrynus regularis. The Retention factor (Rf) and
molecular weights of the protein
